As biocatalysts, enzyme is characterized in high catalytic efficiency, high specificity, mild reactive conditions and controllable activity. 酶作为生物催化剂,具有高催化效率,专一性强,反应条件温和及酶活性可以调控。
Isomerism is very important because enzyme specificity restricts viable organisms to the use of only one of the two forms. 异构现象是非常重要的,因为酶的特异性限制生物体都只能使用两种同分异构体中的一种。
As one of the biocatalyst, enzyme have excellent properties of high selectivity and specificity, activity, reaction conditions are moderate, many countries have paid great attention for a long time. 酶作为生物催化剂,因具有专一性强、催化效率高、反应条件温和等特点,长期以来,受到世界各国重视;
The advantage of chemical-enzyme synthesis is high enzyme specificity but the disadvantage is higher cost of zymolysis. 化学-酶联合法专一性强,但发酵成本较高。
In conclusion, there is a quantitative relationship between structural properties and enzymatical modification, which is related to the enzyme specificity. 总之,改性蛋白质的结构性质变化与酶作用之间存在明显的定量关系,而且结构性质的变化还与酶特异性有关。
The quality of an enzyme immunoassays depends very much on the purity of the antigen or hapten used for conjugation, the specificity of the antibody and the choice of a suitable enzyme label. 酶免疫分析技术的质量依赖于抗原的纯度、抗体的特异性、合适标记酶的选用,其灵敏度取决于标记酶的高度纯化和高转化率。
The enzyme had better specificity for ramie retting. 该酶对苎麻脱胶有较好的特异性。
Conclusion Competitive PCR combining with enzyme linked hybridization of amplified products has been proven to be a method of choice for quantitative detection of HBV DNA with high sensitivity, specificity and reliability. 结论竞争PCR及其产物酶联杂交定量法是一种相对精确、灵敏度很高和特异性较强的HBV基因全定量测定技术,优于外参照法及其它产物定量法。
These include altering the reaction mechanism of the enzyme to catalyse new reactions, switching substrate specificity, expanding substrate specificity, and improving substrate specificity, such as enantioselectivity in kinetic resolutions. 它包括改变酶反应机制来催化新的反应,改变底物专一性、扩大底物专一性、改善底物专一性,例如动力学拆分中的对映选择性。
In most of the cases, microwave irradiation does not reduce enzyme's activity and improves reaction rate instead, but influences the enzyme specificity. 在大多数场合,适当的微波辐射不会损伤酶活而且可以提高反应速率,而对酶特异性的影响则不一而论。
Amylase is a biocatalyst and the enzyme specificity decides that it has no dis-advantageous effect on its own intensity. 淀粉酶是一种生物催化剂,酶的专一性决定其不会对纤维自身的强度造成不利的影响。
The enzyme catalyzes the esterification of other alcohol with acetyl-COA to form acetate ester, but shows specificity to form phenethyl acetate. 此酶也能催化其它重要乙酸酯的合成,但对乙酸苯乙酯的合成具有特异性。
APAAP bridge-linked enzyme labelling method was established for detection of HIV antibody. Compared with immunofluorescence test ( IF), these two methods had the same specificity and reproducibility, but APAAP is more sensitive for detection of HIV antibody than IF test. 将APAAP桥联酶标技术用于HIV抗体检测,其特异性和重复性与免疫荧光技术相同,敏感性高于后者。
Although many related enzyme properties are well known, the crucial factors in product specificity determination remain to be answered. 目前,人们对这类酶的性质已经有了较为深入的认识,但还存在着一些尚未解决的问题,如酶产物专一性的决定因素尚未系统阐明等。
The tumor markers in peritoneal lavage for peritoneal metastasis from gastric carcinoma are performed by using immunocytochemistry with the monoclonal antibody and chemiluminescent enzyme immunoassay, IFCCs have a positive predictive value of 91% and a specificity of 97% for gastric cancer recurrence. 应用免疫组化和酶联免疫吸附试验可检测腹腔灌洗液(PLF)中IFCCs肿瘤标志,IFCCs预测胃癌复发的阳性预报值为91%,特异性为97%。
So far, all the examples of enzyme redesign have focused on changing substrate or cofactor specificity or altering the stereochemistry of a reaction, but no body has demonstrated alteration of reaction chemistry. 目前,酶的再设计都集中在改变酶分子的底物或辅因子的专一性及反应的立体专一性,而鲜有改变反应的化学专一性的报道。
This assay showed high sensitivity by taking advantage of sample enrichment and enzyme amplification, and exhibited good specificity by involving the selective aptamer binding and the specific enzyme reaction. 利用样品富集和酶催化放大,实现了对凝血酶的高灵敏检测,而适配体的选择性结合和酶的特异性反应使方法具有高特异性。
Biological enzyme possesses the properties of high efficiency, specificity and mild function conditions. The scouring process using biological enzyme instead of alkaline meets the basic requirements of energy-saving emission reduction. 生物酶具有高效、专一、作用条件温和的特点,由生物酶代替烧碱的酶精练工艺符合节能减排的基本要求。
The analyses of SDS-PAGE indicated that different enzyme had different enzyme specificity. Under the condition of low hydrolysis degree, the modes of enzymatic hydrolysis for soy protein were different. 凝胶电泳分析表明,不同酶的催化特异性不同,在低水解程度下,蛋白的酶解模式不同。
Polyclonal antibody can inhibit the enzyme activity to some extent. Double-antibody sandwich enzyme-linked immunosorbent assay establish in research possesse better repeatability and specificity. The optimal detection time for ESBLs is culture for 4h. 多克隆抗体可抑制酶蛋白抗药活性;双抗体夹心酶联免疫吸附试验检测酶蛋白具有较好的重复性、特异性;临床分离菌株产超广谱β-内酰胺酶最佳检测时间为菌株培养4h。
The enzyme exhibited unique phosphoryl donor specificity with maximal activity towards pyrophosphate. 该酶具有独特的磷酸供体底物特异性且最大活性磷酸供体为焦磷酸。
Pullulanase ( Pullulanase, EC. 3.2.1.41) is a debranching enzyme that can hydrolyze pullulan specificity and amylopectin branch points on the α-1, 6 glycosidic bonds. 普鲁兰酶(Pullulanase,EC.3.2.1.41)是脱支酶的一种,它能专一性水解普鲁兰多糖和支链淀粉分支点上的α-1,6糖苷键,在淀粉糖化工业中具有广阔的应用前景。